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Study on the Effects of Oleanolic Acid on the Proliferation ,Invasion and Metastasis of Human Ovarian Cancer SKOV3 Cells and Its Mechanism / 中国药房
China Pharmacy ; (12): 1190-1197, 2020.
Article in Chinese | WPRIM | ID: wpr-821605
ABSTRACT
OBJECTIVE:To investigate the effects and m echanism of oleanolic acid on inhibiting the proliferation ,invasion and metastasis of human ovarian cancer SKOV 3 cells. METHODSCCK-8 assay was used to detect the effects of different concentrations of oleanolic acid (10,20,40,60,80,100 μmol/L)on the proliferation of ovarian cancer SKOV 3 cells at 12,24, 36 and 48 h. The effects of low-dose and high-dose of oleanolic acid (20,40 μmol/L)on the metastasis and invasion ability of SKOV3 cells for 24 h were observed in Transwell assay. Western blotting assay was used to detect the effects of low-dose and high-dose of oleanolic acid on the protein expression of NF-κB p65,PRL-3,TNF-α,IL-6 and E-cadherin in SKOV 3 cells. Through LPS induction and NF-κB p65 plasmid transfectionWestern blotting and RT-qPCR assay were used to investigate the effects of low-dose and high-dose oleanolic acid on the expression of NF-κB/PRL-3 pathway related proteins and their mRNA. RESULTS : With the increase of the concentration and action time of oleanolic acid ,the proliferation capacity of ovarian cancer SKOV 3 cells was decreased ,the surval rates of administration groups were significantly lower than that of the control group (P<0.05 or P< 0.01). Low-dose and high-dose of oleanolic acid could significantly reduce the number of migrating and invading cells (P<0.05 or P<0.01). The protein relative expression of NF-κB p65,PRL-3,TNF-α and IL-6 in SKOV 3 cells were significantly decreased , while the protein relative expression of E-cadherin was significantly increased (P<0.05 or P<0.01). After LPS induction ,protein and mRNA relative expression of NF-κB p65,PRL-3,TNF-α and IL-6 were increased significantly in LPS model group ,while protein and mRNA relative expression of E-cadherin were significantly decreased (P<0.05 or P<0.01). The protein and mRNA relative expression of NF-κ B p65,PRL-3,TNF-α and IL-6 were significantly decreased ,and protein and mRNA relative expression of E-cadherin were significantly increased in low-dose and high-dose of oleanolic acid group (P<0.05 or P<0.01). In SKOV3 cells with over-expressed NF-κB p65,low-dose and high-dose of oleanolic acid c ould significantly down-regulat the proteinexpression of NF-κ B p65,PRL-3,TNF-α and IL-6,while upregult the protein relative expression of E-cadherin (P<0.05 E-mail:122821905@qq.com or P<0.01). CONCLUSIONS : Oleanolic acid can inhibit SKOV3 cells proliferation,invasion and metastasis by regulating NF-κB/PRL-3 signaling pathway.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2020 Type: Article