Your browser doesn't support javascript.
loading
Effect of Baicalin on myocardial cell apoptosis and Akt/AMPK/mTOR signal pathway expression in chronic myocardial failure rats / 医学研究生学报
Journal of Medical Postgraduates ; (12): 577-581, 2020.
Article in Chinese | WPRIM | ID: wpr-821811
ABSTRACT
ObjectiveTo investigate the effect of Baicalin on the apoptosis of cardiomyocytes and the expression of Akt/Amp activated protein kinase (AMPK)/mammalian rapamycin target protein (mTOR) signaling pathway in rats with chronic myocardial failure.MethodsSixty male SD rats of SPF grade were randomly divided into normal group, model group, positive control group and experimental group, with 15 rats in each group. Rats in the positive control group were given 40mg/kg losartan once a day. Rats in the experimental group were given 50mg/kg Baicalin once a day. Rats in the normal group and the model group were given the same dose of saline once a day. Each group was given gavage for 4 weeks. HE staining was used to observe the pathomorphology of cardiomyocytes. The myocardial tissue index (CAI) was detected by in situ end labeling. The expressions of Bax and bcl-2 protein in myocardium were detected by immunohistochemistry. The cardiac function was detected by echocardiography. The expression of Akt, AMPK and mTOR mRNA was detected by RT-PCR.ResultsCompared with the normal group, the apoptosis indexes of the model group, the positive control group and the experimental group were increased (P0.05). Compared with the model group, the expression of Bcl-2 protein was increased and Bax protein was decreased in the positive control group and the experimental group (P0.05). Compared with the normal group, the LVEFs of the model group, the positive control group and the experimental group were decreased, while the LVD and LVS were increased (P0.05).ConclusionBaicalin can alleviate myocardial injury in rats with chronic myocardial failure, and its mechanism may be related to the upregulation of Akt/AMPK/mTOR signaling pathway.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Medical Postgraduates Year: 2020 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Medical Postgraduates Year: 2020 Type: Article