1-beta-D-Arabinofuranosyl-cytosine Induces Chromosomal Breaks in vitro
Journal of the Korean Pediatric Society
;
: 1186-1193, 2003.
Article
in Korean
| WPRIM
| ID: wpr-82187
ABSTRACT
PURPOSE:
Fragile sites are points on chromosomes which tend to break non-randomly when exposed to specific chemical agents or conditions of tissue culture. The chromosomal break induced by the antineoplastic drug, 1-beta-D-arabinofuranosyl-cytosine(Ara-c), was investigated to study the laboratory conditions in which the incidence of chromosomal break could be enhanced. Besides, the fragile sites induced by Ara-C were investigated and compared to the already known locations of the specific chromosomal alterations observed in specific neoplasms.METHODS:
T-lymphocytes from theree normal males and three females were cultured for 48 hours. Cells from each individual were exposed to the Ara-C for an additional 24 hours. After the caffeine was added during the last six hours culture, the metaphase chromosomes were prepared following the conventional method. A site was considered fragile if it was found to break two or more per 100 chromosomal breaks in more than four of six individuals tested.RESULTS:
Ara-C induced 252.1 chromosomal breaks per 100 mitotic cells and this result was significantly higher than that of the control, which induced 25.2 breaks(P<0.05). The incidence of the chromosomal break by Ara-C was higher, if cultured in the MEM-FA, which has no folic acid, than in the RPMI 1640 which contains enough folic acid(P<0.05). The most common break site by Ara-C was 3p14.2(FRA3B). There were 20 fragile sites induced by Ara-C. Among these 20 fragile sites, seven coincided with the locations of the mapped oncogenes, JUN, SKI, REL, N-MYC, FHIT, MET, ETS-1, and FOS.CONCLUSIONS:
S phase specific chemotherapeutic agent, Ara-C, induced the expression of the chromosomal fragile sites effectively using the T-lymphocyte in vitro. Some of the fragile sites by Ara-C highly coincided with the oncogenes and neoplasm specific chromosome breakpoints. In this regard, the fragile sites reported here could provide the unknown neoplasm related chromosomal alternation points.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oncogenes
/
Caffeine
/
T-Lymphocytes
/
Incidence
/
S Phase
/
Genes, jun
/
Chromosome Breakage
/
Cytarabine
/
Chromosome Breakpoints
/
Folic Acid
Type of study:
Incidence study
/
Prognostic study
Limits:
Female
/
Humans
/
Male
Language:
Korean
Journal:
Journal of the Korean Pediatric Society
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS