miRNA-325-3p reduces the radio-sensitivity of nasopharyngeal carcinoma CNE1 cells by down-regulating the expression of cytokeratin 13 / 中国肿瘤生物治疗杂志

ABSTRACT

@#[Abstract] Objective： ：To investigate the effect of miRNA-325-3p and its target gene cytokeratin 13 (CK13) on the radio-sensitivity of nasopharyngeal carcinoma cell line CNE1. Methods： ：The potential target gene of miRNA-325-3p was predicted by three databases miRBase, Targetscan and microcosm, and verified by Double luciferase activity assay. QPCR was used to detect the expression levels of miRNA-325-3p and its target gene in nasopharyngeal carcinoma cell line CNE1 under different radiation doses; To verify the changes in radio-sensitivity of nasopharyngeal carcinoma cells, colony formation assay, Flow cytometery and MTT were used to observe the clone formation, apoptosis and cell viability of CNE1 cells after overexpression of miRNA-325-3p and knockdown of CK13 under different radiation doses, respectively. Results： ：CK13 was confirmed as a potential target gene of miRNA-325-3p. After radiotherapy, the expression level of miRNA-325-3p in CNE1 cell was significantly increased, while the expression level of CK13 was decreased (all P<0.05); up-regulation of miRNA-325-3p expression and silence of CK13 gene increased cell survival rate (upregulation of miRNA325-3p [60.14±3.55]% vs [19.23±3.42]%, t=14.37, P<0.01; silence of CK13 [76.15±5.13]% vs [28.53±3.68]%, t=13.06, P<0.01) and colony formation rate, and decreased apoptosis rate (upregulation of miRNA-325-3p [27.95±2.67]% vs [51.68±3.47]%, t=9.39, P<0.01; silence of CK13 [20.31±2.62]% vs [38.14±3.83]%, t=6.66, P<0.01). Conclusion： ：miRNA-325-3p can reduce the sensitivity of nasopharyngeal carcinoma cell line CNE1 to radiotherapy by down-regulating the target gene CK13.