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Comparative Study on the Contents of Four Compounds from Different Processed Products of Miao Medicine Chimonanthus nitens and Its Toxicity / 中国药房
China Pharmacy ; (12): 1475-1462, 2020.
Article in Chinese | WPRIM | ID: wpr-822368
ABSTRACT
OBJECTIVE: To compare the contents of 4 components and toxicity from Miao medicine Chimonanthus nitens samples after drying in the shade ,oven drying ,steam,charring. METHODS :The contents of scopolin,fraxin,scopoletin and isofraxin were simultaneously determined by HPLC. The separation was performed on Welch-C18 column with mobile phase consisted of acetonitrile-water (gradient elution )at the flow rate of 1 mL/min. The detection wavelength was set at 214 nm,and column temperature was 30 ℃,and the sample size was 10 μL. Modified Karber method was used to determine the LD 50 and its 95%CI of different processed C. nitens to mice and evaluate its acute toxicity. RESULTS : The linear range of scopolin , fraxin, scopoletin and isofraxin were 0.019-1.856,0.016-1.616,0.009-0.920,0.006-0.624 μg(R2 were all not lower than 0.999 5). RSDs of precision , stability and reprodu-cibility tests were all lower than 2.0%(n=6). The average recoveries were 104.49%,102.22%, 101.45%,99.26%(all RSDs were lower than 2%,n=6). The contents of scopolin were 1.119 0%,0.904 3%, 1.068 4%and 0.036 4%;those o f fraxin were 0.867 8%,0.453 9%,0.423 7%and 0.020 5%;those of scopoletin ; those of isofraxidin were 0.110 2%,0.202 1%,0.208 1% and 0.249 4%in samples after drying in the shade ,oven CX-2018-001) drying, steam, charring. The LD 50 of 4 processedproducts were 4 118.13,3 733.36,1 643.61,>10 000 qq.com mg/kg samples ;95%CI were (3 748.87,4 523.76), (3 422.16,4 072.86),(1 520.90,1 776.23),(>10 000) mg/kg. CONCLUSIONS :HPLC method is reproducible and precise. It can be used to determine the contents of 4 components in different processed products of C. niten s. The contents of 4 components in different processed products , and the contents of glycosides toxicity components are decreased significantly. All the 4 processed products were low or non-toxic.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2020 Type: Article