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Comparison of Chemical Constituents Differences between Angelicae dahuricae with and without Sulphur- fumigation and Selection of Chemical Markers Based on 1H-NMR Metabolomics / 中国药房
China Pharmacy ; (12): 1557-1561, 2020.
Article in Chinese | WPRIM | ID: wpr-822619
ABSTRACT
OBJECTIVE:To study the diff erences in the overall chemical constituents of Angelicae dahuricae with and without sulphur-fumigation,and to select chemical markers that can be used for identification. METHODS :Each 16 batches of A. dahuricae with and without sulphur-fumigation were selected as samples. Deuterated methanol-deuterium oxide (1 ∶ 0.2,V/V)was used as extraction solvent ,and 1H-NMR metabolomics was used to detect the primary and secondary metabolites in A. dahuricae . In addition ,visual analysisprincipal component analysisPCA)and t-test were used to compare the component differences of A. dahuricae by two kinds of pretreatment methods. RESULTS :A total of 19 chemical constituents such as coumarinsamino acids and sugars were simultaneously detected and identified from two kinds of A. dahuricae . Visual analysis showed that the chemical profiles of the two kinds of A. dahuricae were similar ,but their coumarins and carbohydrates were quite different in 1H-NMR signal intensity. PCA analysis showed that all samples could be divided into two categories according to different treatment methods. The results of t-test showed that the contents of oxypeucedanin ,imperatorin,glucose and sucrose of A. dahuricae were decreased significantly after sulphur fumigation (P<0.01 or P<0.001),while the contents of alanine and leucine were significantly increased (P<0.01 or P<0.001). There was no statistical significance in the contents of other 13 kinds of components (P>0.05). CONCLUSIONS:There are obvious differences in the 6 chemical compounds of oxypeucedanin ,imperatorin,glucosesucrosealanine and leucine in A. dahuricae with and without sulphur-fumigation ,which can be used as chemical markers for the identification of sulphur-fumigated A. dahuricae in the market.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2020 Type: Article