Comparative Genetic Characterization of Plasmids of Agrobacterium Species Isolated in Korea / 영남의대학술지
Yeungnam University Journal of Medicine
;
: 41-48, 1984.
Article
in Korean
| WPRIM
| ID: wpr-82266
ABSTRACT
The soil bacterium Agrobacterium tumefaciens is a plant pathogen that causes crown gall tumors by infecting the wounded dicotyledonous plants and subsequent integration of bacterial DNA into plant nuclear DNA. Virulent A. tumefaciens strains harbor a large Ti (tumor–inducing) plasmid that carries genes essential for tumorigenesis. In the present study, 13 strains (Malus pumila Mill; A₁₋₃, Populus monilifera; W₁₋₆, Populus tomentiglandlosa; P₁₋₃ and Rosa species; R₁) of Agrobacterium isolated in korean crown gall tumors and plasmids were observed in 6 strains (W₂, W₃, W₆, P₁, P₃ and A₂). The test for crown gall tumor formation was resulted only in ATCC15955 and KW2 strains inoculated into the stem of sun flower and the development was observed for 4 and 6 weeks after inoculation. Above two Ti plasmids (pTi) were purified by cesium chloride-ethidium bromide density gradient centrifugation and digested with restriction enzyme and fragments of pTiATCC15955 and pTiKW₂ observed by EcoR I ; 25&27, Hind III ; 23&21, BamH I ; each 20 and Hpa I ; 12&27. And sizes of pTiATCC15955 and and pTiKW₂ calculated as 200 and 87 kbases. Octopine was isolated from tumor tissue (W₁₋₆ and P₁₋₃) and these strains confirmed as octopine type.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plant Tumors
/
Plants
/
Plasmids
/
Soil
/
Wounds and Injuries
/
DNA
/
DNA, Bacterial
/
Centrifugation, Density Gradient
/
Cesium
/
Solar System
Country/Region as subject:
Asia
Language:
Korean
Journal:
Yeungnam University Journal of Medicine
Year:
1984
Type:
Article
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