Retention Time and Ion Abundance Ratio of 8 Common Drugs (Poisons) by LC-MS/MS Qualitative Analysis / 法医学杂志
Journal of Forensic Medicine
;
(6): 216-222, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-823438
ABSTRACT
Objective To investigate the maximum allowable deviations of retention time and ion abundance ratio of the 8 common drugs (poisons) from 3 categories, poisons (methamphetamine, morphine, ketamine), benzodiazepines (estazolam, midazolam, diazepam, clonazepam) and barbiturates (phenobar-bital) in blood, by liquid chromatograpy-tandem mass spectrometry (LC-MS/MS) in forensic toxicology analysis. Methods The deviations of retention time and ion abundance ratio at 7 low mass concentrations, limit of detection (LOD), 2LOD, limit of quantitation (LOQ), 1.5LOQ, 2LOQ, 4LOQ and 6LOQ, were tested by LC-MS/MS after liquid-liquid extraction under the conditions of two chromatographic columns and three chromatographs. Results The deviation of absolute retention time of 98.11% of 8 drugs (poisons) in the blood samples was within the range of ± 0.05 min, and that of the relative retention time of 96.21% was within the range of±0.4%. The maximum deviation of the ion abundance ratio was highly correlated with the mass concentration. When the mass concentration of drugs (poisons) was LOQ or above, more than 95% of the absolute deviation and relative deviation of the ion abundance ratio were in the range of±25% and±40%, respectively; when the mass concentration was below LOQ, the range could be expanded to±35% and±50%, respectively. Conclusion It is recommended for the determination range of the absolute retention time deviation of 8 common drugs (poisons) to be±0.1 min and that of the relative retention time deviation to be±1.0%. The determination range of absolute deviation of the ion abundance ratio should be±25% when the mass concentration is LOQ or above, and the relative deviation should be±40%. When the mass concentration is below LOQ, the deviation determination range can be expanded to±35% and±50%, respectively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Qualitative research
Language:
Chinese
Journal:
Journal of Forensic Medicine
Year:
2020
Type:
Article
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