Methanolic extract of Clausena excavata promotes wound healing via anti-inflammatory and anti-apoptotic activities
Asian Pacific Journal of Tropical Biomedicine
;
(12): 232-238, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-823937
ABSTRACT
Objective:
To investigate the anti-inflammatory properties of methanolic extract of Clausena excavata in lipopolysaccharide (LPS)-activated macrophages (J774A.1) and the effect on skin wound in a rat model through determining cytokine levels and gene expressions.Methods:
The effects of methanolic extract of Clausena excavata on in vitro viability and TNF-α, IL-6, IL-10, and nitric oxide release by LPS-activated J774A.1 cells were determined. In addition, relative expressions of BAX, BCL-2 and COX-2 genes were examined in healed wounds of rats.Results:
The methanolic extract of Clausena excavata was not toxic to J774A.1 cells at the highest dose of 400 μg/mL. It decreased levels of TNF-α and IL-6, while increasing IL-10 level in LPS-activated J774A.1 cells and in the healed wounds of rats. The methanolic extract of Clausena excavata also inhibited nitric oxide production in LPS-activated J774A.1 cells. The BAX and COX-2 genes were downregulated while the BCL-2 gene was upregulated in the healed wound of rats.Conclusions:
The methanolic extract of Clausena excavata promotes wound healing via its anti-inflammatory and anti-apoptotic activities.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Asian Pacific Journal of Tropical Biomedicine
Year:
2020
Type:
Article
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