Evaluation of the T-KB-H and 3-HB Kits for the Measurement of Serum Ketone and beta-Hydroxybutyric Acid
Laboratory Medicine Online
;
: 22-27, 2014.
Article
in Korean
| WPRIM
| ID: wpr-82416
ABSTRACT
BACKGROUND:
Diabetes mellitus and alcohol consumption are the most common causes of ketoacidosis in adults. Recently, beta-hydroxybutyric acid (betaHBA) was reported to be a potential serum biomarker in the diagnosis and monitoring of ketoacidosis. We evaluated the performance of T-KB-H and 3-HB kits for the measurement of ketone bodies [acetoacetate (AcAc)+betaHBA] and betaHBA, respectively.METHODS:
Quantitative enzymatic assays were performed using the T-KB-H and 3-HB kits (Nittobo Medical Co., Japan) and the Architect ci16200 Integrated System (Abbott Laboratories, USA). Simultaneously, the ketone body levels in these serum samples were determined by gas chromatography-mas spectrometry (GC-MS). We evaluated precision and linearity of these kits and correlation with GC-MS, and established reference intervals in children and adults.RESULTS:
The coefficients of variation for the T-KB-H and 3-HB kits were less than 4.0% at analyte levels of 50, 100, and 400 micromol/L. Linearity was observed for AcAc and betaHBA over a 0-1,000 micromol/L range (R2<0.99). Results from the T-KB-H and 3-HB kits were in good agreement with those from the GC-MS analysis, with correlation coefficients of 0.94 for AcAc and 0.96 for betaHBA. Reference intervals determined for the T-KB-H kit were 9.8-270.1 micromol/L and 18.5-531.8 micromol/L in children and adults, respectively. For the 3-HB kit, the reference intervals were 6.4-234.0 micromol/L and 16.0-437.2 micromol/L in children and adults, respectively.CONCLUSIONS:
The T-KB-H and 3-HB kits displayed good precision, clinically acceptable linearity, and reliable correlation with an established assay. This indicates that the kits can be used clinically for measuring serum ketone bodies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spectrum Analysis
/
Alcohol Drinking
/
Diabetic Ketoacidosis
/
3-Hydroxybutyric Acid
/
Diabetes Mellitus
/
Diagnosis
/
Enzyme Assays
/
Ketone Bodies
/
Ketosis
/
Gas Chromatography-Mass Spectrometry
Type of study:
Diagnostic study
Limits:
Adult
/
Child
/
Humans
Language:
Korean
Journal:
Laboratory Medicine Online
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS