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Effects of oxygen glucose deprivation and reperfusion on gap junction and glucose uptake of astrocytes / 中华行为医学与脑科学杂志
Chinese Journal of Behavioral Medicine and Brain Science ; (12): 967-970, 2019.
Article in Chinese | WPRIM | ID: wpr-824249
ABSTRACT
Objective To explore the relationship between gap junction and glucose uptake of astrocytes under oxygen-glucose deprivation(OGD) and reperfusion.Methods Cerebral cortical astrocyte from 1 day newborn SD rats were undergone the primary culture.The ischemia cell model was established by OGD.This experiment were divided into control group,OGD group and OGD + CBX group.After different reperfusion time (0 h,12 h 24 h and 48 h),the glucose uptake of astrocyte was measured by 2-NBDG through flow cytometry analysis and connexin 43(Cx43) gap junction plaques was detected using immunofluorescene.Results Compared with the control group,the glucose uptake of astrocyte was up-regulated induced by OGD following different reperfusion time.The glucose uptake of OGD group was (2.32 ± 0.43)nmol/μgDNA in 24 hours reperfusion time and was (0.95±0.28)nmol/μgDNA in control group.The upregtulation was up to 2.63-fold increase (t=13.99,P=0.0024) in 24 hours after reperfusion.Compared with the control group,the Cx43 gap junction number was up to 2.5-fold increase(t=11.34,P=0.003) and the size was 1.85-fold increase (t=10.27,P=0.004) in 24 h reperfusion.The glucose uptake of astrocyte after OGD was reduced by CBX and the decrease was 42% in 48 h after reperfusion.Conclusion Those results urges us consider the clinical treatment for interfering with Cx43 gap junction.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Behavioral Medicine and Brain Science Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Behavioral Medicine and Brain Science Year: 2019 Type: Article