Rhizoma Pleionis inhibits of breast cancer epithelial-mesenchymal transition by VEGF-A related angiogenesis / 中国医师杂志

ABSTRACT

Objective To investigate the Rhizoma Pleionis mediating vascular endothelial growth factor A (VEGF-A)-related angiogenesis in breast cancer cells to inhibit epithelial-mesenchymal transition (EMT) in breast cancer.Methods Firstly,the proliferation activity of MDA-MB-231 and 4T1 of breast cancer cells was detected by methyl thiazolyl tetrazolium (MTT) method at different concentrations (0.2 g/ml,0.4 g/ml,0.6 g/ml,0.8 g/ml,1.0 g/ml);secondly,the specific concentration of Rhizoma Pleionis was selected and tested by Transwell test for its effect on breast cancer cell metastasis and invasion.The formation of human umbilical vein endothelial cells (HUVEC) capillary wall was measured to detect the effect of Rhizoma Pleionis on cell angiogenesis.The EMT-related protein and angiogenesis-related molecules were detected by Western blot.Results MTT resuhs showed that Rhizoma Pleionis of 0.8 g/ml and 1.0 g/ml can inhibit the MDA-MB-231 and 4T1 cells proliferation in a dose-dependent manner,with statistically significant difference (P ＜ 0.05).Rhizoma Pleionis can inhibit the invasion of breast cancer cells and inhibit the formation of blood vessel walls in HUVECs.Western blot confirmed that the expression level of EMT-related proteinsE-cadherin was significantly increased and the expression level of N-cadherin,Vimentin and Snail was significantly decreased after treatment with Rhizoma Pleionis (P ＜ 0.05);and the level of the related molecule hypoxia inducible factor-1 α (HIF-1 α),VEGF-A and vascular endothelial growth factor receptor-2 (VEGFR-2) was significantly reduced (P ＜ 0.05).Conclusions Rhizoma Pleionis inhibits the epithelial-mesenchymal transition of breast cancer by influencing the angiogenesis of VEGF-A,and inhibits the invasion and metastasis of breast cancer.