The expression and clinical significance of DDR2 and IFITM1 in nasopharyngeal carcinoma / 中国医师杂志

ABSTRACT

Objective To investigate the expression of discoid domain receptor 2 (DDR2) and interferon-induced transmembrane protein 1 (IFITM1) in nasopharyngeal carcinoma (NPC) patients and their clinical significance.Methods From November 2014 to November 2015,65 patients with nasopharyngeal carcinoma were selected.All patients underwent nasopharyngeal biopsy.All specimens were embedded in paraffin after biopsy.All specimens were confirmed by pathology.Another 65 rhinitis mucosa specimens with chronic inflammation confirmed by pathology in the same period were selected as control group.The clinical and pathological data of the two groups were collected.The mRNA and protein expression levels of DDR2 and IFITM1 in nasopharyngeal carcinoma tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry.The correlation between them was analyzed by Pearson.All patients were followed up for 3 years,and the 3-year survival period of the patients was analyzed by Kaplan-Meier method.Results The results of qRT-PCR showed that the relative expression levels of DDR2 and IFITM1 in nasopharyngeal carcinoma tissues were significantly higher than those in control group (P ＜0.05).Immunohistochemical results showed that the positive rates of DDR2 and IFITM1 in nasopharyngeal carcinoma were significantly higher than those in control group (P ＜ 0.05).The results of expressions of DDR2 and IFITM1 and the clinicopathological analysis of the patients showed that the expression of DDR2 was correlated with TNM stage,differentiation degree and infiltration depth,and IFITM1 was correlated with lymph node metastasis,TNM stage,differentiation degree and infiltration depth (P ＜ 0.05).Pearson correlation analysis showed that there was correlation between DDR2 and IFITM1 (r =0.608,P ＜ 0.01).Kaplan-Meier analysis showed that the progression-free survival rate (41.17％) and total survival rate (52.94％) in DDR2 negative expression group were significantly higher than those in positive expression group (16.67％,18.75％,P ＜ 0.05).The progression-free survival rate (42.86％) and total survival rate (61.90％) in IFITM1 negative expression group were significantly higher than those in positive expression group (18.18％,20.45％,P ＜0.05).Conclusions DDR2 and IFITM1 are highly expressed in nasopharyngeal carcinoma,which may be involved in the occurrence and development of nasopharyngeal carcinoma and significantly affect the prognosis of patients.