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Effects of exogenous ghrelin on muscle atrophy in elderly mice / 中华老年医学杂志
Chinese Journal of Geriatrics ; (12): 1404-1407, 2019.
Article in Chinese | WPRIM | ID: wpr-824579
ABSTRACT
Objective To investigate the effects of acylated ghrelin and des-acylated ghrelin on skeletal muscle atrophy in elderly mice.Methods Eighteen-month-old wild type (WT)mice and ghrelin-/-mice were selected to perform body composition analysis and wheel-running test under conditions of feeding versus fasting.The gene expressions of myogenic regulatory factors including muscle differentiation factor MyoD,myogenin,atrogin-1,muscle-specific RING finger protein 1 (muRF-1),and insulin growth factor 1 (IGF-1) in mice gastrocnemius muscle were detected by realtime polymerase chain reaction (PCR).Results The locomotor activity during the wheel-running test were significantly lower in elderly ghrelin-/-mice than in elderly WT mice (3 929 ± 263 times/h vs.5359± 601 times/h,t =4.87,P < 0.05).The gene expressions of MyoD,myogenin,atrogin-1,muRF-1 and IGF-1 had no significant difference between the two groups (P > 0.05).After 48 h fasting,the decrements of body weight,fat and muscle weight were more in ghrelin-/-mice than in WT mice(P<0.05).In fasting old ghrelin-/-mice,the gene expressions of MyoD and myogenin were increased(improved) (t =232.00 and 121.00,P < 0.05),and the gene expressions of atrogin-1 and muRF-1 were decreased(improved) (t =30.40 and 54.00,P<0.05)after treatment with both acylated ghrelin and desacylated ghrelin.Conclusions The acylated ghrelin and desacylated ghrelin may play protective roles in age-related muscle atrophy.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Geriatrics Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Geriatrics Year: 2019 Type: Article