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Dynamic changes in peripheral type Ⅱ innate lymphoid cell ( ILC2 ) subpopulation and its clinical significance in children with hay fever during the pollen season / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology ; (12): 25-31, 2020.
Article in Chinese | WPRIM | ID: wpr-824820
ABSTRACT
Objective To analyze the dynamic changes in the expression and function of peripher-al typeⅡinnate lymphoid cell (ILC2) subpopulation and the activity of signal transducers and activators of transcription (STAT6) in children with hay fever during pollen season. Methods A total of 10 patients with hay fever, 10 patients with house dust mite ( HDM)-sensitized asthma and 12 healthy controls ( HC) were enrolled in this study. Changes in peripheral ILC2 and the intracellular expression of Th2-related cyto-kines were detected by flow cytometry during and outside the pollen season. Peripheral Lin- cell population was isolated from each group and cultured with the presence of IL-25 or IL-33 for 7 d. The concentrations of IL-5 and IL-13 in culture supernatants were measured by ELISA. Expression of phospho-STAT6 at protein level was quantified by Western blot. Results Within the pollen season, the percentage of peripheral ILC2 cells was significantly higher in children with hay fever [(23. 09±7. 86)%] than in children with HDM-sen-sitized asthma [(6. 84±3. 85)%, P<0. 05] and healthy children[(1. 69±0. 87)%, P<0. 05]. In the non-pollen season, the peripheral ILC2 cells in children with hay fever presented a decreasing trend [(11. 30±2. 45)%], but was still higher than that in HDM-sensitized asthmatics [(3. 76±1. 96)%, P<0. 05] and HC [(1. 32±0. 91)%, P<0. 05] at the same time point. Moreover, peripheral IL-13+ILC2 cells in children with hay fever [(6. 94±3. 16)% vs(4. 17±1. 98)%, P<0. 05] and in HDM-sensitized asthmatics [(1. 89 ±0. 70)% vs(1. 44±0. 55)%, P<0. 05] during the pollen season were significantly higher than those in the non-pollen season. After the in vitro stimulation with IL25 or IL-33, the levels of IL-5 and IL-13 in culture supernatants were both increased in children with hay fever and HDM-sensitized asthmatics, and a synergis-tic action was observed when IL25 and IL-33 were used in combination. Meanwhile, the protein level of phospho-STAT4 in Lin-cells was significantly up-regulated in the hay fever group after stimulation with IL25 and IL-33. Conclusions During the pollen season, the abnormal number and function of ILC2 subpopula-tion in children with hay fever might be another cause of the occurrence of clinical symptoms in a short period of time or acute exacerbation.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Microbiology and Immunology Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Microbiology and Immunology Year: 2020 Type: Article