Expression pattern research of Cyclin D1 in scored human lens epithelial cells cultured with high glucose / 国际眼科杂志(Guoji Yanke Zazhi)
International Eye Science
;
(12): 1699-1703, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-825325
ABSTRACT
@#AIM:
To observe the expression pattern of Cyclin D1 in human lens epithelial cells(HLECs)after traumatic stimulation in high-glucose culture<p>METHODS:
The activity of HLECs was detected by MTT method after incubate with differernt concentration glucose for 24h <i>in vitro </i>to determine the optimal glucose concentration. qRT-PCR and Western blot were used to detect the high glucose pretreatment group and the non-high glucose pretreatment group. The expression of Cyclin D1 in HLECs at different time points after traumatic stimulation was detected.<p>RESULTS:
The viability of HLECs were increased when treatment with low concentration glucose, but the concentration should not exceed 25.5mmol/L, or it will inhibit the activity of HLECs; The reasult of high glucose pretreatment group reveal that the expression of Cyclin D1 is down-regulated in a time-dependent manner within a certain time range. While the expression of Cyclin D1 was irregular in the non-pretreatment group, it was increased at the time point of 12h and 48h. The score treatment can up-regulate the expression of Cyclin D1 in HLECs in a certain degreen.<p>CONCLUSION:
The effects of glucose on HLECs activity and Cyclin D1 experssion are irrugular. Trauma treatment can stimulate the expression of Cyclin D1 in HLECs to some extent.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
International Eye Science
Year:
2020
Type:
Article
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