lncRNA LINC00969 inhibits breast cancer cell proliferation and migration / 中国肿瘤生物治疗杂志

ABSTRACT

@#[Abstract] Objective: To investigate the effect of long non-coding LINC00969 on proliferation and invasion of breast cancer cell. Methods: Real-time Quantitative polymerase chain reaction (qPCR) was used to detect differential expression of LINC00969 in five breast cancer cell lines (MCF-7, BT-20, MAD-MB-231, ZR-75-1, and SKBR3) , normal breast cells MCF-10A, and in 42 cases breast cancer tissues and adjacent tissues. TMCF-7 cells were transfected with LINC00969 plasmid and empty vector vector vector. The transfection efficiency was verified by qPCR. CCK-8, plate cloning and edu assay were used to detect cell proliferation. Flow cytometry was used to detect cell cycle. Western blotting was used to detect PCNA, CyclinD1, MMP2 and MMP9. Scratch repair test and Transwell test were used to detect cell migration and invasion. Results：Compared with the adjacent tissues, LINC00969 expression in breast cancer tissues was significantly decreased (P<0.05); compared with breast cancer cells MCF-10A, LINC00969 expression in five breast cancer cells was significantly decreased (P<0.05), and the lowest was in MCF-7 cells; overexpression of LINC00969 significantly inhibited the proliferation, colony formation and DNA synthesis of MCF-7 cells (all P<0.05), making MCF-7 cell cycle clear.The ability of wound healing, migration and invasion of the cells were significantly reduced (P<0.05). Overexpression of LINC00969 significantly inhibited the expression of PCNA, cyclinD1, MMP2 and MMP9 (all P<0.05). Conclusion: LINC00969 is low expressed in breast cancer. Overexpression of LINC00969 can inhibit proliferation and migration of breast cancer cell,the mechanism may be related to the abnormal expression of cell cycle and migration related proteins.