In vitro anticancer activity of polysaccharide extracted from red alga Jania rubens against breast and colon cancer cell lines

ABSTRACT

Objective:To evaluate the potential role of the polysaccharides of the marine algae as an anticancer agent in vitro against colon cancer cell line (CoCa2) and breast cancer (MCF7) cell lines and to measure lactate dehydrogenase enzyme (LDH) activity as biomarker of membrane integrity of the cells.Methods:The cells of breast cancer (MCF7) and colon cancer (CoCa2) were used to evaluate the potential anticancer role of the polysaccharides of marine algae. Anti-proliferative activity against MCF7 and CoCa2 cell lines were evaluated in vitro by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.Results:The in vitro assay of the antioxidant activity of eight marine seaweed species showed that the red seaweed Jania rubens (J. rubens) had the highest DPPH (2,2 diphenyl-1-picrylhydrazyl) free radical scavenging activity. The extracted polysaccharides with concentrations 0.1–40.0 mg/mL from J. rubens were tested for its anticancer potentiality and cytotoxic effects against the cell lines of human breast (MCF7) and colon cancer (CoCa2) cell lines by MTT assay. The inhibitory concentration at 50 (ICConclusions:The polysaccharides of the red marine alga J. rubens could be a potential candidate for the natural compounds as antioxidant and anticancer therapy.