MiR-144 Inhibits the Malignant Biologic Behaviors of Multiple Myeloma Cells by Regulating Wnt4/β-Catenin Signaling Pathway / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1256-1260, 2020.
Article
in Zh
| WPRIM
| ID: wpr-827130
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the effect of miR-144 to the biological behavior of multiple myeloma cells and its mechanism.@*METHODS@#RT-PCR was used to detect the expression of miR-144 in multiple myeloma cells and plasma of MM patients. MTT assay was used to detect the proliferation and cloning ability of myeloma cells transfected by miR-144. Flow cytometry was used to detect the cell cycle distribution of myeloma cells with over-expression of miR-144. Apoptosis of myeloma cells with over-expression of miR-144 was detected by TUNEL assay. Transwell cell invasion and migration assay was used to detect the invasion and migration ability of myeloma cells with overexpressing on miR-144.Western blot analysis was used to detect the protein expression levels of MMP-9 and MMP-2 in myeloma cells with over expression of miR-144, as well as the expression levels of proteins related to Wnt/β-catenin signaling pathway.@*RESULTS@#The expression level of miR-144 in MM cell lines and blood of MM patients was significantly lower than that in control group (P<0.05). The proliferation, invasion and migration of myeloma cells with over-expression of miR-144 were significantly decreased (P<0.05), and the apoptosis level was increased (P<0.05). The expression levels of MMP-9, MMP-2, Wnt/β-catenin signaling pathway in myeloma cells with over-expression of miR-144 were significantly lower than those in control group (P<0.05).@*CONCLUSION@#MiR-144 can inhibit the proliferation, migration and invasion of multiple myeloma cells and induce cell apoptosis. The specific mechanism may be related with the activity of inhibiting Wnt/β-catenin signaling pathway.
Full text:
1
Index:
WPRIM
Main subject:
Biological Products
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Gene Expression Regulation, Neoplastic
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Cell Movement
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Apoptosis
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MicroRNAs
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Cell Line, Tumor
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Cell Proliferation
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Beta Catenin
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Wnt4 Protein
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Wnt Signaling Pathway
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2020
Type:
Article