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Effect of Quercetin on Proliferation and Apoptosis of Multiple Myeloma Cells and Its Related Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1234-1239, 2020.
Article in Chinese | WPRIM | ID: wpr-827134
ABSTRACT
OBJECTIVE@#To investigate the effect of quercetin (que) on proliferation and apoptosis of multiple myeloma cell line NCI-H929.@*METHODS@#NCI-H929 cells were routinely cultured, and cells in logarithmic growth phase were taken and used for experiments. After treatment of NCI-H929 cells with Que of 50, 100 and 200 µmol/ L for 24, 48 and 72 hours, the proliferation level of cells was detected by using MTT method; after treatment of NCI-H929 cells with Que of 100 and 200 µmol/ L for 24 hours, the cell apoptosis level was detected by Annexin V-FITC/PI double staining, the changes of cell cycle was analysis by flow cytometry with PI marking; the expression of apoptosis-related proteins caspase-3, caspase-8, caspase-9, PARP, BCL-2 and cell cycle-related proteins P53, P21, P27, CDK4, and the activiation of ERK and ATK were detected by Western blot.@*RESULTS@#Que of different concentration could inhibit cell proliferation with time and dose dependent manner. The flow cytometry showed that Que could significantly increase the cell apoptosis and arrest NCI-H929 cells in the G/M phase. In addition, Western blot analysis showed that Que could activate the apoptosis-related proteins, such as caspase-3, caspase-8, caspase-9 and PARP, and then inhibited the expression of BCL-2. Que could promote the expression of P53, P21 and P27, however, it could inhibited the CDK4 expression in NCI-H929 cells. Que could decrease the phosphorylation levels of p-ERK and p-AKT in NCI-H929 cells.@*CONCLUSION@#Quercetin mediates anti-myeloma effects through inducing apoptosis, cell cycle arrest and down-regulating ERK and AKT pathways in human myeloma cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Quercetin / Apoptosis / Cell Line, Tumor / Cell Proliferation / Multiple Myeloma Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Quercetin / Apoptosis / Cell Line, Tumor / Cell Proliferation / Multiple Myeloma Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2020 Type: Article