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Inhibitory Effect of S1PR2 Antagonist JTE-013 on Proliferation of Chronic Myeloid Leukemia Cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 1081-1085, 2020.
Article in Chinese | WPRIM | ID: wpr-827157
Responsible library: WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of sphingosine-1-phosphate receptor 2 (S1PR2) specific antagonist JTE-013 on the proliferation of human chronic myeloid leukemia (CML) cell line K562.@*METHODS@#K562 cells were treated with JTE-013 (0, 0.5, 1, 5, 10, 20 μmol/L) for 24 and 48 hours respectively, CCK8 assay was used to detect the cell viability. K562 cells were treated with JTE-013 (0, 5, 10, 20 μmol/L) for 24 hours, propidium iodide (PI) DNA staining was used to analyze the cell cycle, Western blot was used to determine the levels of P21 and Cyclin D1 protein expression.@*RESULTS@#JTE-013 inhibited the proliferation of CML cell line K562 in a dose dependent manner (r=-0.971). The proliferation rate of CML cells showed that the activity of CML cells decreased gradually with the increase of JTE-013 concentration (r=-0.971). The detection demonstrated that JTE-013 suppressed tumor cell proliferation through cell cycle arrest in G/G phase. Further detection of the protein expressions of G phase regulators showed that level of P21 increased, and expression of Cyclin D1 decreased.@*CONCLUSION@#JTE-013, a S1PR2 antagonist, can inhibit the proliferation of human CML K562 cells, which may be achieved by arresting the cells in G/G phase.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pyrazoles / Pyridines / Humans / Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Apoptosis / K562 Cells / Receptors, Lysosphingolipid / Cell Proliferation / Sphingosine-1-Phosphate Receptors Language: Chinese Journal: Journal of Experimental Hematology Year: 2020 Type: Article
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pyrazoles / Pyridines / Humans / Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Apoptosis / K562 Cells / Receptors, Lysosphingolipid / Cell Proliferation / Sphingosine-1-Phosphate Receptors Language: Chinese Journal: Journal of Experimental Hematology Year: 2020 Type: Article