Nagilactone E increases PD-L1 expression through activation of c-Jun in lung cancer cells / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 517-525, 2020.
Article
in English
| WPRIM
| ID: wpr-827217
ABSTRACT
Nagilactone E (NLE), a natural product with anticancer activities, is isolated from Podocarpus nagi. In this study, we reported that NLE increased programmed death ligand 1 (PD-L1) expressions at both protein and mRNA levels in human lung cancer cells, and enhanced its localization on the cell membrane. Mechanistically, NLE increased the phosphorylation and expression of c-Jun, and promoted the localization of c-Jun in the nucleus, while silencing of c-Jun by small interfering RNA (siRNA) reduced NLE-induced PD-L1. Further study showed that NLE activated the c-Jun N-terminal kinases (JNK), the upstream of c-Jun, and its inhibitor SP600125 reversed the NLE-increased PD-L1. Moreover, NLE-induced PD-L1 increased the binding intensity of PD-1 on the cell surface. In summary, NLE upregulates the expression of PD-L1 in lung cancer cells through the activation of JNK-c-Jun axis, which has the potential to combine with the PD-1/PD-L1 antibody therapies in lung cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
English
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2020
Type:
Article
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