Your browser doesn't support javascript.
loading
Simultaneous rapid detection of ten stilbenes in serum of mice by UPLC-MS/MS / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-827964
Responsible library: WPRO
ABSTRACT
Stilbenes is a class of natural polyphenols with 1,2-diphenylethylene as the skeleton structure which have structural and active diversity. However, there are fewer studies on their metabolic process, which limits the in-depth research and development of such components. An UPLC-MS/MS method simultaneously determining contents of ten stilbenes was firstly established in this study and applied to study the ten stilbenes of peony seed coats in the serum of C57 mice.Piceatannol was the internal standard, and methanol was used for protein precipitation, UPLC-MS/MS with negative ion mode was used for analysis, and the method was validated.The serum samples were collected and detected after mice being oral administered with 800 mg·kg~(-1) peony seed coat extracts for 8 weeks. The results showed that suffruticosol A, suffruticosol B, suffruticosol C, trans-ε-viniferin, cis-gnetin H, trans-suffruticosol D and trans-gnetin H were detected in serum samples, and the highest is suffruticosol A. The method is simple and quick with high specificity and sensitivity, and it is suitable for quantitative determination of ten stilbenes in the serum of mice.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Seeds / Stilbenes / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Chromatography, Liquid / Paeonia / Tandem Mass Spectrometry / Animals / Mice Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2020 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Main subject: Seeds / Stilbenes / Chemistry / Reproducibility of Results / Chromatography, High Pressure Liquid / Chromatography, Liquid / Paeonia / Tandem Mass Spectrometry / Animals / Mice Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2020 Type: Article