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Simultaneous determination of four components of Shuganning Injection in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 2626-2633, 2020.
Article in Chinese | WPRIM | ID: wpr-828036
ABSTRACT
This study aims to reveal the pharmacokinetics of Shuganning Injection in normal rats. In this experiment,ultra-high performance liquid chromatography-electrospray-tandem mass spectrometry( UPLC-ESI-MS/MS) was used to establish an analytical method for simultaneous determination of chlorogenic acid,gardenioside,oroxylin A and baicalin in rat plasma. Then,the non-compartmental model( NCA) in Phoenix WinN onL in 6. 4 software was used to fit pharmacokinetic parameters. The methodological validation showed that the linear relationship of the components in rat plasma samples were good( r>0. 995). The recovery rate and matrix effect of plasma samples with low,middle and high concentration were 79. 14%-101. 4%. The intra-day and inter-day precision,accuracy and stability meet the requirements of biological sample analysis. The half-life( t1/2) of chlorogenic acid,gardenioside,oroxylin A did not change significantly and the area under blood concentration-time curve( AUC0-t) is proportional to the dose,which suggested that three components showed a linear kinetic characteristics,but baicalin showed nonlinear kinetic characteristics. Moreover,the retention time of each component in rats was short. The established UPLC-MS/MS quantitative analysis method is rapid,sensitive and accurate,which can be used for the determination of chlorogenic acid,gardenioside,oroxylin A and baicalin in rat plasma and pharmacokinetic study of Shuganning Injection.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasma / Chlorogenic Acid / Reproducibility of Results / Chromatography, High Pressure Liquid / Chromatography, Liquid / Rats, Sprague-Dawley / Tandem Mass Spectrometry Type of study: Prognostic study Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasma / Chlorogenic Acid / Reproducibility of Results / Chromatography, High Pressure Liquid / Chromatography, Liquid / Rats, Sprague-Dawley / Tandem Mass Spectrometry Type of study: Prognostic study Limits: Animals Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2020 Type: Article