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Effect of Huaier aqueous extract on growth and metastasis of human non-small cell lung cancer NCI-H1299 cells and its underlying mechanisms / 中国中药杂志
Article in Chinese | WPRIM | ID: wpr-828395
Responsible library: WPRO
ABSTRACT
This study aims to investigate the effect of Huaier aqueous extract on the growth and metastasis of human non-small cell lung cancer NCI-H1299 cells and its underlying mechanisms. MTT assay was used to detect the effect of Huaier aqueous extract on the proliferation of NCI-H1299 cells. Flow cytometry was used to examine the effect of Huaier aqueous extract on the apoptosis, cell cycle, and ROS level of NCI-H1299 cells. Wound healing assay was used to evaluate the effect of Huaier aqueous extract on the migration ability of NCI-H1299 cells. Western blot was used to detect the levels of proteins involving apoptosis, epithelial-mesenchymal transition(EMT), and MAPK signaling pathway in NCI-H1299 cells exposed to Huaier aqueous extract. The results showed that Huaier aqueous extract inhibited the proliferation of NCI-H1299 cells, and induced cell-cycle arrest at the phase S. Huaier aqueous extract promoted the apoptosis of NCI-H1299 cells by down-regulating the expression of anti-apoptotic protein Bcl-2. Moreover, Huaier aqueous extract increased ROS level and induced ferroptosis in NCI-H1299 cells. EMT played a critical role in cancer metastasis. Huaier aqueous extract reduced the migration ability of NCI-H1299 cells by inhibiting EMT of NCI-H1299 cells. In addition, this study revealed that Huaier aqueous extract inhibited MAPK signaling pathway in human non-small cell lung cancer NCI-H1299 cells, which may be one of Huaier's mechanisms in inhibiting growth and metastasis of NCI-H1299 cells. This study provides a new theoretical basis for the clinical treatment of lung cancer with Huaier, and important reference significance for further studies on the anti-tumor mechanisms of Huaier.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Humans / Apoptosis / Carcinoma, Non-Small-Cell Lung / Cell Line, Tumor / Complex Mixtures / Cell Proliferation / Trametes / Lung Neoplasms Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Humans / Apoptosis / Carcinoma, Non-Small-Cell Lung / Cell Line, Tumor / Complex Mixtures / Cell Proliferation / Trametes / Lung Neoplasms Language: Chinese Journal: China Journal of Chinese Materia Medica Year: 2020 Type: Article