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LC-MS/MS analysis of pharmacokinetics of 3, 29-dibenzoyl karounitriol in rats following administration of Trichosanthes sustained-release pellets / 浙江大学学报·医学版
Zhejiang Daxue xuebao. Yixue ban ; (6): 995-1000, 2020.
Article in Zh | WPRIM | ID: wpr-828521
Responsible library: WPRO
ABSTRACT
OBJECTIVE@#To study the plasma concentration and pharmacokinetics of 3, 29-Dibenzoyl Karounitriol (3, 29-DK) from sustained- release pellets and extracts of Trichosanthes at different time points in rats using high-performance liquid chromatography- tandem mass spectrometry (LC-MS/MS).@*METHODS@#Healthy male SD rats were given a single gavage of Trichosanthes sustained-release pellets or Trichosanthes extract, and orbital blood samples were taken at different time points within 48 h after drug administration in the pellet group and within 5 h in Trichosanthes extract group for determination of the plasma concentrations of 3, 29-DK using LC-MS/MS. The standard curve of 3, 29-DK content was established, and the specificity, minimum detection limit, precision and accuracy, extraction recovery, stability and matrix effect of LC-MS/MS analysis were assessed. The mean plasms levels of 3, 29-DK at different time points after the drug administration were determined and its pharmacokinetic parameters were calculated using Das 2.0 software.@*RESULTS@#LC-MS/MS analysis showed a good linearity of 3, 29-DK concentration within the range of 0.5-32 ng/mL, and the results of methodological validation confirmed the validity of this method for biological sample determination. Trichosanthes sustained-release pellets and Trichosanthes extract showed significant differences in their AUC, AUC, MRT, MRT, t and T of 3, 29-DK after administration in rats ( < 0.05).@*CONCLUSIONS@#Trichosanthes sustained-release pellets are capable of sustained-release of 3, 29-DK in rats, and thus provides a basis for the study of new dosage forms of Trichosanthes.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Benzoates / Pharmacokinetics / Chemistry / Reproducibility of Results / Chromatography, Liquid / Rats, Sprague-Dawley / Area Under Curve / Trichosanthes / Delayed-Action Preparations / Tandem Mass Spectrometry Type of study: Prognostic_studies Limits: Animals Language: Zh Journal: Zhejiang Daxue xuebao. Yixue ban Year: 2020 Type: Article
Full text: 1 Index: WPRIM Main subject: Benzoates / Pharmacokinetics / Chemistry / Reproducibility of Results / Chromatography, Liquid / Rats, Sprague-Dawley / Area Under Curve / Trichosanthes / Delayed-Action Preparations / Tandem Mass Spectrometry Type of study: Prognostic_studies Limits: Animals Language: Zh Journal: Zhejiang Daxue xuebao. Yixue ban Year: 2020 Type: Article