Effect of vitreous cavity transplantation of bone marrow mesenchymal stem cells on optic nerve protection in rat glaucoma model / 国际眼科杂志(Guoji Yanke Zazhi)

ABSTRACT

@#AIM:To investigate the neuroprotective effect of vitreous cavity transplantation of bone marrow mesenchymal stem cells on rat glaucoma model. <p>METHODS: Rat primary bone marrow mesenchymal stem cells were extracted and identified; Lewis rats were randomly divided into 3 groups Control group, Glaucoma group and bone marrow mesenchymal stem cell group(BMMSCs group), and rat glaucoma model were made the left eye was the experimental eye(glaucoma), the right eye is the control eye, and identified them. Then vitreous cavity transplantation of bone marrow mesenchymal stem cells were performed; HE staining was used to observe the morphology of the retina; The number of retinal ganglion cells was detected by immunofluorescence; TUNEL staining was used to observe the apoptosis of retinal ganglion cells; The expression of IGF1 and BDNF protein in retinal tissue was detected by Western blot. <p>RESULTS: The intraocular pressure of the experimental eyes of the rats was significantly higher than that of the control eyes(<i>P</i><0.01), indicating that the glaucoma rat models were successfully constructed; In the Glaucoma group, the retinal nerve fibers were irregularly arranged, the number of retinal ganglion cells and retinal thickness were significantly lower than that of the control group(<i>P</i><0.01), and the number of apoptosis of retinal ganglion cells was significantly higher than that of the control group(<i>P</i><0.001); The retinal nerve fiber cells in the BMMSCs group were arranged neatly. The number of retinal ganglion cells and retinal thickness were significantly higher than those in the Glaucoma group(<i>P</i><0.05), and the number apoptosis of retinal ganglion cells was significantly lower than that of Glaucoma group(<i>P</i><0.01); The expression of IGF1 and BDNF in the retina of the Glaucoma group was significantly lower than that in the control group(<i>P</i><0.01), the expression of IGF1 and BDNF in the retina of the BMMSCs group was higher than that in the Glaucoma group(<i>P</i><0.05). <p>CONCLUSION:Bone marrow mesenchymal stem cell vitreous cavity transplantation can improve glaucoma in rats and protect retinal ganglion cells.