Effects of ciliary neurotrophic factor over-expression by adenovirus on bone marrow mesenchymal stem cells / 国际眼科杂志(Guoji Yanke Zazhi)

ABSTRACT

@#AIM: To establish the model of bone marrow mesenchymal stem cells(BMSCs)over-expressing ciliary neurotrophic factor(CNTF)by applying adenovirus. It may provide novel strategies for <i>in-vitro </i>investigations of retinopathies.<p>METHODS: GFP-adenovirus and CNTF-adenovirus were synthesized then used to transfect BMSCs of passage 3. Blank control group(without adenovirus transfected group), negative control group(GFP-adenovirus transfected group), and experimental group(CNTF-adenovirus transfected group)were included in this study. On the 1, 2, 3d post-transfected, ELISA assay was applied to examine CNTF protein-secretion in the supernate.<p>RESULTS: GFP-adenovirus and CNTF-adenovirus models were successfully established. The CNTF protein levels in the supernate were higher in experimental group than those in the blank control group and negative control group(<i>P</i><0.05). <p>CONCLUSION: CNTF-modified BMSCs by adenovirus could efficiently secrete CNTF protein <i>in-vitro</i>.