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Study on Optimization of the Nine-time Repeat Steaming and Sun-drying Process Processing Technology of Polygonum multiflorum / 中国药房
China Pharmacy ; (12): 2713-2719, 2020.
Article in Chinese | WPRIM | ID: wpr-829971
ABSTRACT
OBJECTIVE:To establish methods for the content determination of stilbene glycosideemodinemodin methyl ether and total polysaccharide in P. multiflorum ,and to optimize the processing technology of traditional nine-time repeat steaming and sun-drying process of P. multiflorum . METHODSHPLC method was adopted to determine the contents of stilbene glycosideemodin,and emodin methyl ether. The content of total polysaccharide was determined by UV spectrometry. With steaming time , drying time and drying temperature as independent variables ,the contents of stilbene glycosideemodinemodin methyl ether and total polysaccharide as indexes ,the processing technology of nine-time repeat steaming and sun-drying process of P. multiflorum was optimized by weighted comprehensive score method and central composite design-response surface method ,and the validation tests were conducted. RESULTS :The linear range of sample size of stilbene glycosideemodin and emodin methyl ether were 0.27-2.7 µg(r=0.997 1),0.063-0.63 µg(r=0.999 9),0.038-0.38 µg(r=0.990 9),respectively. The linear range of mass concentration of total polysaccharide was 2.07-12.42 µg/mL(r=0.999 6),respectively. RSDs of precision ,stability and reproducibility tests were all lower than 2%. The recoveries were 99.43%-101.06%(RSD=0.63%,n=6),98.74%-120.33% (RSD=1.34%,n=6),98.39%-102.44%(RSD=1.49%,n=6),99.51%-101.98%(RSD=0.87%,n=6),respectively. The optimal processing technology was steaming time for 4.5 h,drying time for 9 h,drying temperatrue for 66 ℃ and repeat processing for 9 times. Results of 3 times of validation tests showed that the comprehensive score of optimal technology was 35.69, relative error of which to predicted value (36.90)was 3.30%. CONCLUSIONS :Established method is simple and reproducible , and the optimal processing technology is predicive ,stable feasible.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: China Pharmacy Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: China Pharmacy Year: 2020 Type: Article