Long-Term Effects of Diesel Exhaust Particles on Airway Inflammation and Remodeling in a Mouse Model
Allergy, Asthma & Immunology Research
;
: 246-256, 2016.
Article
in English
| WPRIM
| ID: wpr-83198
ABSTRACT
PURPOSE:
Diesel exhaust particles (DEPs) can induce and trigger airway hyperresponsiveness (AHR) and inflammation. The aim of this study was to investigate the effect of long-term DEP exposure on AHR, inflammation, lung fibrosis, and goblet cell hyperplasia in a mouse model.METHODS:
BALB/c mice were exposed to DEPs 1 hour a day for 5 days a week for 3 months in a closed-system chamber attached to a ultrasonic nebulizer (low dose 100 microg/m3 DEPs, high dose 3 mg/m3 DEPs). The control group was exposed to saline. Enhanced pause was measured as an indicator of AHR. Animals were subjected to whole-body plethysmography and then sacrificed to determine the performance of bronchoalveolar lavage and histology.RESULTS:
AHR was higher in the DEP group than in the control group, and higher in the high-dose DEP than in the low-dose DEP groups at 4, 8, and 12 weeks. The numbers of neutrophils and lymphocytes were higher in the high-dose DEP group than in the low-dose DEP group and control group at 4, 8, and 12 weeks. The levels of interleukin (IL)-5, IL-13, and interferon-gamma were higher in the low-dose DEP group than in the control group at 12 weeks. The level of IL-10 was higher in the high-dose DEP group than in the control group at 12 weeks. The level of vascular endothelial growth factor was higher in the low-dose and high-dose DEP groups than in the control group at 12 weeks. The level of IL-6 was higher in the low-dose DEP group than in the control group at 12 weeks. The level of transforming growth factor-beta was higher in the high-dose DEP group than in the control group at 4, 8, and 12 weeks. The collagen content and lung fibrosis in lung tissue was higher in the high-dose DEP group at 8 and 12 weeks.CONCLUSIONS:
These results suggest that long-term DEP exposure may increase AHR, inflammation, lung fibrosis, and goblet cell hyperplasia in a mouse model.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plethysmography
/
Pneumonia
/
Ultrasonics
/
Vehicle Emissions
/
Fibrosis
/
Nebulizers and Vaporizers
/
Lymphocytes
/
Collagen
/
Interleukins
/
Interleukin-6
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Allergy, Asthma & Immunology Research
Year:
2016
Type:
Article
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