Your browser doesn't support javascript.
loading
NanoString nCounter® Approach in Breast Cancer: A Comparative Analysis with Quantitative Real-Time Polymerase Chain Reaction, In Situ Hybridization, and Immunohistochemistry / 한국유방암학회지
Journal of Breast Cancer ; : 286-296, 2017.
Article in En | WPRIM | ID: wpr-83452
Responsible library: WPRO
ABSTRACT
PURPOSE: Accurate testing for estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) is essential for breast cancer treatment. At present, immunohistochemistry (IHC)/florescence in situ hybridization (FISH) are widely accepted as the standard testing methods. To investigate the value of NanoString nCounter®, we performed its comparative analysis with IHC/FISH and real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) for the assessment of ER, PR, and HER2. METHODS: Data on IHC/FISH results for ER, PR, and HER2 in 240 patients from a single tertiary hospital in Korea were collected and compared with NanoString nCounter® and qRT-PCR results at a single institution. RESULTS: Expression levels for each gene using NanoString nCounter® showed good correlation with the corresponding data for protein expression by IHC (p<0.001) and gene amplification status for HER2 (p<0.001). Comparisons between gene expression and IHC data showed good overall agreement with a high area under the curve (AUC) for ESR1/ER (AUC=0.939), PgR/PR (AUC=0.796), and HER2/HER2 (AUC=0.989) (p<0.001). CONCLUSION: The quantification of ER, PgR, and HER2 mRNA expression with NanoString nCounter® may be a viable alternative to conventional IHC/FISH methods.
Subject(s)
Key words
Full text: 1 Index: WPRIM Main subject: Breast / Breast Neoplasms / RNA, Messenger / Immunohistochemistry / Receptors, Progesterone / Gene Expression / Gene Amplification / Polymerase Chain Reaction / In Situ Hybridization / Reverse Transcription Type of study: Prognostic_studies Limits: Humans Country/Region as subject: Asia Language: En Journal: Journal of Breast Cancer Year: 2017 Type: Article
Full text: 1 Index: WPRIM Main subject: Breast / Breast Neoplasms / RNA, Messenger / Immunohistochemistry / Receptors, Progesterone / Gene Expression / Gene Amplification / Polymerase Chain Reaction / In Situ Hybridization / Reverse Transcription Type of study: Prognostic_studies Limits: Humans Country/Region as subject: Asia Language: En Journal: Journal of Breast Cancer Year: 2017 Type: Article