Effects of Cryopreservation and Thawing on Single-Cell Transcriptomes of Human T Cells
Immune Network
;
: e34-2020.
Article
| WPRIM
| ID: wpr-835449
ABSTRACT
Cryopreservation and thawing of PBMCs are inevitable processes in expanding the scale of experiments in human immunology. Here, we carried out a fundamental study to investigate the detailed effects of PBMC cryopreservation and thawing on transcriptomes. We sorted Tregs from fresh and cryopreserved/thawed PBMCs from an identical donor and performed single-cell RNA-sequencing (scRNA-seq). We found that the cryopreservation and thawing process minimally affects the key molecular features of Tregs, including FOXP3. However, the cryopreserved and thawed sample had a specific cluster with up-regulation of genes for heat shock proteins. Caution may be warranted in interpreting the character of any cluster of cells with heat shock-related properties when cryopreserved and thawed samples are used for scRNA-seq.
Full text:
Available
Index:
WPRIM (Western Pacific)
Journal:
Immune Network
Year:
2020
Type:
Article
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