Neiyi recipe improving reproductive ability of mice with endometriosis and its mechanism / 第二军医大学学报

ABSTRACT

Objective To explore the effect of Neiyi recipe on the reproductive ability of mice with endometriosis (EM) and its mechanisms. Methods The EM mouse model was established and divided into Neiyi recipe group (n＝26), gestrinone group (n＝25), EM model group (n＝26), and sham operation group (n＝24). After 15 days of continuous drug intervention, 6 proestrous mice were randomly selected from each group for histomorphological observation. The remaining mice were mated at the ratio of male to female of 41 for 15 days. The pregnancy, endometriosis, pelvic adhesions and follicular development were observed and recorded in the 4 groups. The histomorphology of the liver, kidney and spleen of the mice were observed in each group. The concentrations of IL-2, IL-6 and IL-10 in peritoneal fluid of the mice were detected by enzyme-linked immunosorbent assay. Results The pregnancy rate, volume of lesions and pelvic adhesion of the mice were significantly improved in the Neiyi recipe group compared with the gestrinone group and the EM model group (P0.05, P0.01). The ovarian histomorphological observation showed that the follicular development of the mice was better in the Neiyi recipe group than that in the gestrinone group and the EM model group. The mice in the Neiyi recipe group had significantly better outcome in improving follicular development compared with the gestrinone group (P0.05). Compared with the sham operation group, the levels of IL-2 and IL-6 in the peritoneal fluid of the mice were significantly increased in the EM model group (all P0.01), and the level of IL-10 was significantly decreased (P0.05). Compared with the EM model group, the levels of IL-2 in the peritoneal fluid of the mice were significantly decreased in the Neiyi recipe group and the gestrinone group (P0.01), the level of IL-6 in the peritoneal fluid of the mice was significantly decreased in the gestrinone group (P0.05) and the level of IL-10 was significantly increased (P0.01). The pregnancy rate of the mice was not improved in the gestrinone group, and the body mass and the wet mass of liver and kidney were significantly decreased after drug treatment (all P0.05), while the mice in the Neiyi recipe group showed no obvious damages of the liver, spleen or kidney. Conclusion Neiyi recipe can improve the pelvic microenvironment, promote follicular development and improve the reproductive ability of EM mice by inhibiting the growth of EM lesions and reducing pelvic adhesion, with no serious adverse effects and with good safety.