The Neuroprotective Effect of Kefir on Spinal Cord Ischemia/Reperfusion Injury in Rats
Journal of Korean Neurosurgical Society
;
: 335-341, 2015.
Article
in English
| WPRIM
| ID: wpr-83799
ABSTRACT
OBJECTIVE:
The main causes of spinal cord ischemia are a variety of vascular pathologies causing acute arterial occlusions. We investigated neuroprotective effects of kefir on spinal cord ischemia injury in rats.METHODS:
Rats were divided into three groups 1) sham operated control rats; 2) spinal cord ischemia group fed on a standard diet without kefir pretreatment; and 3) spinal cord ischemia group fed on a standard diet plus kefir. Spinal cord ischemia was performed by the infrarenal aorta cross-clamping model. The spinal cord was removed after the procedure. The biochemical and histopathological changes were observed within the samples. Functional assessment was performed for neurological deficit scores.RESULTS:
The kefir group was compared with the ischemia group, a significant decrease in malondialdehyde levels was observed (p<0.05). Catalase and superoxide dismutase levels of the kefir group were significantly higher than ischemia group (p<0.05). In histopathological samples, the kefir group is compared with ischemia group, there was a significant decrease in numbers of dead and degenerated neurons (p<0.05). In immunohistochemical staining, hipoxia-inducible factor-1alpha and caspase 3 immunopositive neurons were significantly decreased in kefir group compared with ischemia group (p<0.05). The neurological deficit scores of kefir group were significantly higher than ischemia group at 24 h (p<0.05).CONCLUSION:
Our study revealed that kefir pretreatment in spinal cord ischemia/reperfusion reduced oxidative stress and neuronal degeneration as a neuroprotective agent. Ultrastructural studies are required in order for kefir to be developed as a promising therapeutic agent to be utilized for human spinal cord ischemia in the future.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta
/
Pathology
/
Spinal Cord
/
Superoxide Dismutase
/
Reperfusion Injury
/
Catalase
/
Oxidative Stress
/
Neuroprotective Agents
/
Spinal Cord Ischemia
/
Cultured Milk Products
Type of study:
Prognostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Journal of Korean Neurosurgical Society
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS