Physicochemical properties and in vitro degradation of a novel collagen scaffold material from basa fish (Pangasisus haniltoa) skin / 第二军医大学学报

ABSTRACT

Objective To prepare a novel collagen scaffold material using Basa fish (Pangasisus haniltoa) skin as the ingredient and to analyze the structural characteristics, physical properties and degradability of the prepared material, so as to explore whether Basa fish can replace terrestrial mammals for preparing a novel collagen scaffold material. Methods Basa fish skins were lyophilized to obtain the membrane material after repeated degreasing, decolorization and dedoping. Crude protein content was determined by the Kjeldahl method. Structure of the materials and its pore size and distribution were analyzed by scanning electron microscopy (SEM). Porosity was measured by the liquid displacement technique, and tensile strength was tested using universal testing machine. The changes of viscosity with temperatures were detected to determine the denaturation temperature of the material.The material was immersed in the phosphate-buffered solution (0.1 mol/L, pH 7.4), which was placed in a constant temperature shaker at 37°, and the water absorption and weight loss rates of the material were detected. Results The crude protein content of the collagen scaffold material was 95.2%, with visually uniform thickness. SEM photographs showed that one side of the material had a rough surface and porous structure, on which varying sizes of pores distributed uniformly; the other side was smooth with dense pores. The porosity of the material was (55.50±1.94)%, thickness was (0.66±0.10) mm and tensile strength was (18.82±0.94) MPa. The denaturation temperature of the material was 34° before thermo-crosslinking and 36° after thermo-crosslinking. The water absorption of the material was (379.77±77.81)% at 48 h. At 28 d after thermo-crosslinking, the degradation rate was (80.22±2.49)%, and the pH value of buffer was 6.67±0.05. Conclusion The collagen scaffold material from Basa fish skin can be made into the biological membrane with uniform thickness, and the membrane comprises double structures dense layer and loose layer. This material exhibits excellent mechanical strength and appropriate denaturation temperature, but its degradation is fast, which needs further improvement.