Development and anti-fatigue function of shenjia chewable tablet / 第二军医大学学报

ABSTRACT

Objective To develop a new type of anti-fatigue nutrient chewable tablet and to explore its anti-fatigue effect in mice. Methods Ginseng polysaccharide, acanthopanax extract and taurine were mixed together in a proportion of 6 8 1 as remedium cardinale, mannitol was used as filler, sucrose as a correctant and magnesium stearate as a lubricant. Hardness, appearance and taste were utilized as score indexes, the dosage of mannitol, sucrose, and magnesium stearate were used as study factors. The orthogonal test was applied to screen the best proportion of raw materials to create the formulation according to the mouse behaviors. A total of 60 male mice were randomly divided into control group (normal saline) and three doses of Shenjia chewable tablet groups (200, 600 and 1 800 mg kg). Lavage administration was lasted for 7 days. We then carried out the weight loading swimming test to record the swimming lime of mice, and also recorded the body mass and the serum biochemical indexes, including adenosine triphosphate (ATP), lactic acid (Lac), free triiodothyronine (FT3). free thyroid hormone (FT4). cortisol and melatonin (MT). Results The formulation was optimized as 30% ginseng polysaccharide, 40% acanthopanax extract, 5% taurine. 19% mannitol, 5% sucrose, and 1. 0% magnesium stearate. The Shenjia chewable tablet tasted cool and sweet, round in shape, yellow in color and smooth in appearance. Compared with the control group, there were significant increases in the loaded-swimming time in the three doses groups (P<0. 01), in the levels of ATP, FT3, FT4 and MT in middle and high dosage groups (P<0. 05, P<0. 01), and in Lac level in high dosage group (PCortisol was increased in the three doses groups (P<0. 05, P<0. 01). All mice in three doses groups had no significant increase in body weight. Conclusion The Shenjia chewable tablet is an ideal anti-fatigue nutrient with reasonable formulation, simple preparation technology, high drug loading and good taste.