Effect of electret electrostatic-field on preparing nanopaticles carrying quaternary ammonium chitosan and epidermal growth factor / 第二军医大学学报

ABSTRACT

Objective To study the effect of electret electrostatic-field on the preparation and characteristics of nanopaticles carrying quaternary ammonium chitosan and epidermal growth factor (EGF), so as to explore the percutaneous transport mechanism of drug-loading nanoparticles. Methods Under different electret electrostatic-fields, ionic crossing method was used to prepare EGF-loading chitosan nanoparticles using EGF as model drug, chitosan as material, and sodium tripolyphosphate (TPP) as crosslinking agent. Transmission electron microscope was employed to observe the surface morphology of nanoparticles * the particle size and the zeta potential of the EGFToading nanoparticles were also examined. Results (1) The EGF-loading chitosan nanoparticles prepared in this study had uniform distribution, good dispersibility and stability. (2) The size of the EGF-loading nanoparticles increased with the increase of chitosan concentration, indicating that chitosan concentrationmight be the main reason for the nanoparticle size. (3) The particle size of the EGF-loading chitosan nanoparticles decreased with the increase of electrostatic field; and the change of the zeta potential was closely related to the polarity of the applied electric field. (4) The particle size of EGFloading nanoparticles decreased with the increase of storage time. Conclusion The morphology, particle size and zeta potential of chitosan nanoparticles are closely related to the intensity and polarity of the applied electric field; proper electric field parameters shouldbe chosen to prepare high quality chitosan nanoparticles with appropriate particle size and stability.