Correlation of homocysteine levels with gene polymorphisms of metabolic enzyme in 438 adults taking physical examination in Shanghai, China / 第二军医大学学报

ABSTRACT

Objective To investigate the correlation of homocysteine (Hey) level with gene polymorphism of Hcy- metabolizing enzymes in healthy adults receiving physical examination in Shanghai, China. Methods Totally 438 participants who were receiving physical examination in Shanghai Ren-ai Hospital were included in this study. The plasma total Hey (tHcy) levels and serum folate levels were measured by enzymatic cycling methods and electrochemiluminescence, respectively. Genetic typing was determined by ligase detection reaction(LDR). The plasma tHcy levels were compared between different genotypes, and the association of plasma tHcy levels with genetic polymorphisms was analyzed. Results The incidence of hyperhomocysteinemia (HHcy) was 28. 54% (125/438) in the present study. There was significant difference in the levels of plasma tHcy among the three genotypes of MTHFR C677T (P<0. 001). Heterozygous genotype CA of MTHFR A1298C was associated with a significantly reduced risk of HHcy (OR=0. 49, 95% CI 0. 26-0. 92,P = 0. 027); heterozygous genotype CT of MTHFR C677T was associated with a significantly increased risk of HHcy (OR=2. 15, 95% CI 1. 06-4. 36, P = 0. 035), and homozygous genotype TT was also associated with a significantly increased risk of HHcy (OR=7. 58, 95% CI 3. 1518. 22, P<0. 001). No correlation was found for HHcy risk with MTR G905A, MTRR A66G, MTRR Ac. 56 + 781C, MTR A2756G, or CBS C551G. Conclusion TT and CT genotype of MTHFR C677T have been found to be the risks for HHcy; CA genotype of MTHFR A1298C may help to decrease the risk to HHcy.