Expression of retinoic acid receptor in the mouse embryonic hematopoiesis / 第二军医大学学报

ABSTRACT

Objective To investigate the expression of retinoic acid receptors (RARs) subtypes RAR-α, RAR-β, RAR-γ and retinal dehydrogenases 1(Ra/dh1), Raldh2 in the mouse embryonic hematopoiesis, so as to discuss the role of different RAR subtypes in embryonic hematopoiesis. Methods The yolk sac (YS) at embryonic day 9. 5 (E9. 5), aortagonad- mesonephros (AGM) region at E10. 5 and E11. 5, fetal liver (FL) at E13. 5, E14. 5 and E17. 5 were harvested from the pregnant mice. The bonemarrow (BM) was also harvested from adult mice. The mRNA levels of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 at the above four different hematopoietic sites were assessed by quantitative reverse transcription polymerase chain reaction (RT PCR). Results The mRNA levels of RAR-α, RAR-β, RAR-γ, RaldhX and Raldh2 at E9. 5 YS, E11. 5 AGM regions and BM were significantly higher than those in other relevant hematopoietic tissues (P<0. 01). Compared with the E10. 5 AGM region, E11. 5 AGM region had significantly increasedmRNA levels of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 (P<0. 01), but the mRNA levels of these genes in FL at E13. 5-E17. 5 were not significantly different. Conclusion The expressions of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 in YS, AGM regions and BM are consistent with the situation of colony forming unit erythroid (CFU-E), suggesting that retinoic acid is closely related to the formation of hematopoiesis in the YS, AGM region and BM in mouse, but has less correlation with the FL in mouse.