Effect of hydrogen-rich water on lymphocyte gene expression profiles of beagle dogs receiving gamma ray irradiation / 第二军医大学学报

ABSTRACT

Objective To study the effect of hydrogen-rich water on lymphocyte gene expression of Beagle dogs exposed to gamma ray (γ ray) using GeneChip technology. Methods Male Beagle dogs were divided into control group, irradiation (IR) group and hydrogen-rich water treatment (HRW + IR) group. GeneChip technology was applied to screen the differentially expressed genes in peripheral blood lymphocyte exposed to 2. 0 Gy60Co γ ray for 6 h. Gene ontology (GO) and Kyoto encyclopedia of gene and genomes (KEGG) databases were used to analyze the bioinformatics of the differentially expressed genes, and real-time quantitative PCR was used to validate the results of GeneChip. Results We screened 4 730 differentially expressed genes with over 2-fold changes in IR group and 4 493 genes inHRW+IR group, and identified 1 606 co-differentially expressed genes in the IR and HRW + IR groups. GO terms of biological process, cellular component and molecular function in the IR group were ten, nine and three, respectively, and in HRW+IR group were fifteen, three and four, respectively. The differentially expressed genes were involved in 19KEGG pathways in the IR group, 24 KEGG pathways in HRW+IR group, and five KEGG pathways in both groups. The PCR results of two genes, selected to validate the GeneChip, were consistent with the GeneChip results. Conclusion Our results indicate that the effect of hydrogen-rich water on lymphocyte gene expression of γ ray-radiated Beagle dogs may involve changes of molecular function, cellular component and biological process, and activation of many signal pathways.