Effect of rapid eye movement sleep deprivation on expression of neuropeptide s in dorsomedial nucleus of rat hypothalamus / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 320-323, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-838853
ABSTRACT
Objective To observe the changes of the neuropeptide S (NPS) expression in dorsomedial nucleus of rat hypothalamus after rapid eye movement (REM) sleep deprivation. Methods Totally 24 adult male rats were evenly randomized into 3 groups normal control group (CC), tank control group (TC) and REM sleep deprivation group (SD). Modified multiple platform method (MMPM) was used to establish REM sleep deprivation model in rats. Immunohistochemistry and hybridization in situ were applied to examine the expression of NPS protein and mRNA in the rat hypothalamus. Results There was no significant differences in the expression of NPS protein or mRNA in rats hypothalamus between CC group and TC group. After REM sleep deprivation, NPS protein and mRNA positive cells in rats hypothalamus in SD group were (27. 86 ± 2. 47) and (25. 75±2. 12), respectively, which were significantly more than those in CC group and TC group (16. 75 ± 2. 12 and 19. 63 ± 1. 85 in CC group, 18. 60±1.60 and 18. 50±1.69 in TC group; P
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Controlled clinical trial
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS