Biomarkers and clinicopathologic features of CpG island methylator phenotype lung cancer / 第二军医大学学报

ABSTRACT

Objective To compare the sensitivity and specificity of the new CpG island methylation phenotype (CIMP) selection marker with the classical CIMP selection marker, and to analyze the clinical pathological features of CIMP lung cancer. Methods Genomic DNA was extracted from 50 cases of lung cancer tissues and the corresponding adjacent normal lung tissues in Changhai Hospital. Methylation statues of new CIMP selction marker (SHISA3, CTSL1, C1ORF103 and TMEM200B) and the classical CIMP selection markers (CACNA1G, IGF2, NEUROG1 and RUNX3) were determined by methylation specific PCR, and the results were analyzed by SPSS software. Results Notable methylation was found in the lung caner tissues, with the methylation levels of the eight studied genes in the lung cancer tissues being significantly higher than those in the adjacent tissues (P=0.014). RUNX3 gene methylation was significantly related to lymph node metastasis and PS score (P=0.017, P=0.018). The methylation rate in lung cancer patients aged over 60 was significantly higher than those aged younger than 60 years old (P=0.031). Smoking showed significant influence on CTSL1 gene methylation (P=0.018). Conclusion CpG island methylation phenotypic lung cancer has unique clinical pathological features. Both the new and classic CIMP markers are of high sensitivity and specificity for the diagnosis of lung cancer.