Breeding and genotype identification of NLRP3 gene knock-out mice / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 555-559, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-839146
ABSTRACT
Objective:
To breed and identify NLRP3 gene knock-out mice.Methods:
The NLRP3 gene knock-out heterozygote mice were bred alone and copulated. The offsprings were to have three genotypes wild genotype, heterozygote genotype and homozygote genotype. Genomic DNA was obtained from each pups and were subjected to PCR and T7 endonuclease 1 to identify the genotype. The homozygote mice were mated with the opposite sex heterozygote mice to obtain more homozygote pups.Results:
Breeding and reproducing were both successful, and we obtained heterozygote genotype and homozygote genotype mice with NLRP3 gene knock-out.Conclusion:
Correct methods of breeding, reproducing and identifying can effectively obtain NLRP3 gene knock-out mice from heterozygote mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2014
Type:
Article
Similar
MEDLINE
...
LILACS
LIS