Expression of miRNA-140 in chondrocytes of patients with early osteoarthritis and its function / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 610-615, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-839155
ABSTRACT
Objective:
To observe miRNA-140 expression in chondrocytes of patients with early osteoarthritis (OA)and the effect of transfecting double-stranded miR-140 (d-miRNA-140) on chondrocyte function.Methods:
Normal and OA chondrocytes (4 weeks and 8 weeks) were collected from rabbit model of early OA (A, B and C group, respectively). Quantitative polymerase chain reaction was used to examine miRNA-140 expression and Western blotting analysis was used to examine Col2a1 and MMP-13 protein expression in each group. Col2a1 and MMP-13 protein expression was also observed in chondrocytes transfected with ds-miR-140.Results:
Compared with group A, miR-140 expressions in group B and C were reduced to 63% and 57%, respectively (P0.05). Compared with group A, Col2a1 mRNA expressions in group B and C were reduced by 52% and 63%, respectively (P<0. 01); while MMP-13 mRNA expressions were up-regulated by 3. 01 and 4. 15 folds, respectively (P< 0. 01). Transfection with ds-miR-140 increased Col2a1 mRNA by 60% and 127% in group B and C, respectively(P<0. 01), and the expressions of MMP-13 mRNA in group B and C were reduced to 54. 53% and 42. 61%, respectively(P<0. 01). The changes of Col2a1 and MMP-13 protein expression were the same as that of mPNA.Conclusion:
miR-140 expression is reduced in early OA cartilage, and transfection with ds-miR-140 may increase Col2a1 expression and reduce MMP-13 expression.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2014
Type:
Article
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