Preparation of polyethylene glycol-modified polyarginine for gene delivery and in vitro evaluation / 第二军医大学学报

ABSTRACT

Objective To modify polyargine (PLR) with polyethylene glycol (PEG) and to observe the effect of PEG modilication on PLR cytotoxicity and efticiency of PLR-mediated RNA interference. Methods1 HNMR was used to characterize PLR-PEG and gel electrophoresis was adopted to determine the siRNA-packing capacity of PLR-PEG. The cytotoxicity of PLR-PEG, cellular uptake and RNA interference efficiency of PLR-PEG/siRNA complexes were investigated using prostate cancer stem-like cells(RC-92a/hTERT). Results1 HNMR results showed the successful synthesis of PLR-PEG. It was found that PEG modiiication decreased cytotoxicity of PLR and reduced cellular uptake of PLR/siRNA complexes, but the reduction of cellular uptake was limited when N/P was high. The modiiication also inhibited the efticiency of PLR-mediated RNA interference, but the influence of PEG moditication was not notable within a certain range. Conclusion PEG-moditied PLR may be a promising vector for gene therapy targeting prostate cancer stem cells.