Doxycycline increases chemosensitivity of ovarian cancer H08910 cells to cisplatin / 第二军医大学学报

ABSTRACT

Objective To investigate the anti-tumor effect of doxycycline against ovarian cancer cells and the underlying mechanism. Methods MTT assay was used to test the tumor cell viability after treated with doxycycline alone or in combination with cisplatin. RT-RCR was used to examine CXCR4 mRNA expression in H08910 cells. Western blotting analysis was used to determine CXCR4 protein expression after doxycycline treatment. Results Treatment with low dose of doxycycline (10 ¡j.g/mL) for 48 hours notably inhibited the proliferation of ovarian cancer cell H08910 (P<0. 01), with the inhibition rate being (70±2) % when doxycycline concentration was at 50 jg/mL. Doxycycline combined with cisplatin had greater inhibitory effect against H08910 cells compared with cisplatin alone at the same concentration. Doxycycline treatment down-regulated CXCR4 expression in H08910 cells. Conclusion Doxycycline has a definite inhibitory effect against proliferation of ovarian cancer H08910 cells, and it can increase the sensitivity of tumor cells to cisplatin, which may involve SDF-1/CXCR4 signaling pathway.