Expression of Ku70 and Bax in hippocampus CA1 following cerebral ischemia/reperfusion injury in diabetic rats / 第二军医大学学报

ABSTRACT

Objeceive To compare the expression of Ku70 and Bax in hippocampus CA1 between normal rats and diabetic rats following cerebral ischemia/reperfusion, so as to explore the roles of Ku70 and Bax in aggravating cerebral ischemial reperfusion mjury nn diabetes. Meehods Totally 72 male SD rats were divided into 3 groups randomly sham operated (SO) group, normoglycemia global cerebral ischemia/reperfusion (NCI) group, and diabetic global cerebral ischemia/reperfusion (DCI) group. The rats in DCI group were treated by streptozodn (STZ) and improved Puliinelii's foue-vessel occlusion method to estabiish diabetic global cerebral ischemia/reperfusion modll. Andanimals in NCI group were not gvven STZ, and other treatments were iimllar to those in the DCI group. Animals in the SO group only had blood vessels exposed. Changes of neuron pathology in hippocampus CA1 were observed by H-E stammg under iight microscopy at 1, 6, 24, and 48 h after lschemial reperfusion; expresioon of Ku70 was detected by immunohistochemistry and Western blotting analyils, and expresioon of Bax was detected by immunohistochemistry. Resules The neuron structure in hippocampus CA1 of rats was damaged nnNCI group, and the density of survival neuronswas significanely lower than that nn the SO group at all studied time ponnts(P<0. 05); the neuron structure damage nn DCI group was more severe, and the demity of sunival neurons was signiticanely lower than that of the NCI group at all studied time points(P<0. 05). The expresioon of Ku70 at 1 and 6 h nn NCI group was signiticanely higher than that in the SO group (P<0. 05), and that at 24 and 48 h was signiticanely lower than that nn the SO group(P<0. 05); Ku70 expression nn DCI group was significanely lower than that nn the NCI group at all studied time ponnts(P<0. 05). Bax expresioon in NCI group was signiticanely higher than that in the SO group at all studied time points(P<0. 05), and that in DCI group was signiticanely higher than that in the NCI group at all studied time ponnts (P<0. 05). Conclusion Diabetes can aggravate global cerebral ischemia/reperfusion injury, which may be related to Ku70 Expression decrease and Bax Expression Increase.