Expression of prostate cancer related long non-coding RNAs in prostate cancer and its biological function / 第二军医大学学报

ABSTRACT

Objective To investigate the expression of prostate cancer related long non-coding RNAs (PCRL) in prostate cancer and its biological function. Methods The expression levels of PCRL in prostatic cancer tissues, adjacent tissues and tissues of other organs were examined by qRT-PCR. The expression levels of PCRL in androgen-dependent (LNCaP-AD), androgen-independent (LNCaP-AI) prostatic cancer cell lines and normal prostate epithelial cells (RWPE-1) were also compared by using qRT-PCR. Finally, the expression levels of androgen receptor were compared between LNCaP-AD and LNCaP-AI prostatic cancer cells by qRT-PCR after siRNA interference of PCRL expression. Results PCRL were highly expressed in the prostate and prostatic cancer tissues. Expression of PCRL was notably higher in LNCaP-AI cells than in LNCaP-AD and RWPE-1 cells. Interference of PCRL expression in LNCaP-AI and LNCaP-AD cells significantly increased androgen receptor level (P<0. 000 1 for LNCaP-AI cells). Conclusion Overexpression of PCRL in prostatic cancer is related to the progression of prostatic cancer; and regulatory relation may exist between PCRL and androgen receptor.