Quantitative analysis of multi-components with single marker in simultaneous determination of 3-n-butylphthalide, sedenenolide, and sedanolide in celery seed extract / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 195-198, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-839555
ABSTRACT
Objective:
To develop a quantitative analysis of multi-components with single marker (QAMS) and validate its accuracy and feasibility for simultaneously determining 3-n-butylphthalide (b), sedenenolide (sen), and sedanolide (san) in celery seed extracts (CSE).Methods:
Three main effective components,3-n-butylphthalide, sedenenolide, and sedanolide, were selected as analytes for evaluating the quality of CSE. The relative correction factors (RCF, f) of 3-n-butylthalide to the other two thalides were calculated fb/sen = 0. 226, fb/san = 0.702. The external standard method was used to determine the title compounds in 6 batches of CSE and the results were compared with those of QAMS.Results:
The RSD of RCF calculated by different instruments was between 4.4%-6.7%. The RSD of RCF calculated by the same instrument with different chromatographic columns was between 2. 3%-3. 6%. The established RCF had a good reproducibility. The quantitative results of three thalides determined by external standard method and QAMS method were not significantly different.Conclusion:
The present method can serve as a new mode to determine multi-components in CSE when standard substances are unavailable.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2013
Type:
Article
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