Effect of tubulin tyrosine ligase like 12 and nitrotyrosine on growth of prostate cancer cells / 第二军医大学学报

ABSTRACT

Objective To study the effect of tubulin tyrosine ligase like 12 (TTLL12) and nitrotyrosine on the growth of prostate cancer cells. Methods TTLL12 and crtubulin nitrotyrosination (N-tubulin) were detected by Western blotting analysis in normal prostate epithelial cell lines PWR1E and RWPE1, and prostate cancer cell line DU145; the proliferation of cells was observed by MTT assay. Results The expression of N-tubulin was significantly lower in DU145 cells than those in PWR1E and RWPE1 cells after treatment with nitrotyrosine (P 0. 05). Conclusion TTLL12 allows an escape of prostate cancer cells from the treatment of nitrotyrosine, leading to the escape of prostate cancer cells from the body's surveillance system and acquirement of their abnormal proliferation.